更新時間:2021-03-26
點擊數:537
描述 DMEM (Dulbecco's Modified Eagle Medium)是一種廣泛使用的基礎培養基,可用于支持很多種類的哺乳動物細胞生長。已經在 DMEM 培養基中培養成功的細胞包括原代成纖維細胞、神經元、膠質細胞、HUVEC 以及平滑肌細胞等原代細胞,還包括 HeLa、293、Cos-7和 PC-12等細胞系。針對廣泛的細胞培養應用,提供了多種組分的 DMEM 改良培養基。使用培養基選擇工具查找適合的配方。
這種 DMEM 的改良方式如下:
包含不含
?高糖?HEPES:
?L-谷氨酰胺?碳酸氫鈉
?酚紅
? 丙酮酸鈉
DMEM 的使用
DMEM 與其他培養基的不同之處在于其含有相當于初始 Eagle's Minimal Essential Medium 4 倍濃度的氨基酸和維生素。DMEM 起初采用低糖 (1 g/L) 和丙酮酸鈉配制,但常常在含/不含丙酮酸鈉的高糖環境下使用。DMEM 不含蛋白質、脂質或生長因子。因此,DMEM 需要補充營養成分,通常需要添加 10% 胎牛血清 (FBS)。DMEM 培養基使用碳酸氫鈉緩沖體系 (3.7 g/L),因此需要 5–10% CO2 的環境來維持生理 pH 值。粉末形式的 Gibco? 細胞培養基在配制時需要補充碳酸氫鈉、調節 pH 并過濾(詳情請參閱實驗方案)。
品牌 | 貨號 | 產品名稱 | 規格 |
GIBCO | 10010023 | PBS, pH 7.4 | 500ml |
GIBCO | 10010049 | PBS, pH 7.5 | 10*500ml |
GIBCO | 11875093 | RPMI 1640 Medium | 500ml |
GIBCO | 11875119 | RPMI 1640 Medium | 10*500ml |
GIBCO | 11965092 | DMEM, high glucose | 500ml |
GIBCO | 11965118 | DMEM, high glucose | 10*500ml |
GIBCO | 11995073 | DMEM, high glucose, pyruvate | 10*500ml |
GIBCO | 11995065 | DMEM, high glucose, pyruvate | 500ml |
GIBCO | 11330032 | DMEM/F-12, HEPES | 500ml |
GIBCO | 11330057 | DMEM/F-12, HEPES | 10*500ml |
GIBCO | 11140050 | MEM Non-Essential Amino Acids Solution (100X) | 100ml |
GIBCO | 11140076 | MEM Non-Essential Amino Acids Solution (100X) | 20*100ml |
GIBCO | 10569044 | DMEM, High Glucose, GlutaMAX?, Pyruvate | 10*500ml |
GIBCO | 10569010 | DMEM, High Glucose, GlutaMAX?, Pyruvate | 500ml |
GIBCO | 10566024 | DMEM, High Glucose, GlutaMAX? | 10*500ml |
GIBCO | 10566016 | DMEM, High Glucose, GlutaMAX? | 500ml |
GIBCO | 22400105 | RPMI 1640 Medium, HEPES | 10*500ml |
GIBCO | 22400089 | RPMI 1640 Medium, HEPES | 500ml |
GIBCO | 15140163 | Penicillin-Streptomycin (10,000 U/mL) | 20*100ml |
GIBCO | 15140122 | Penicillin-Streptomycin (10,000 U/mL) | 100ml |
GIBCO | 25200056 | Trypsin-EDTA (0.25%), phenol red | 100ml |
GIBCO | 25200072 | Trypsin-EDTA (0.25%), phenol red | 500ml |
GIBCO | 25200114 | Trypsin-EDTA (0.25%), phenol red | 20*100ml |
GIBCO | 17504044 | B-27 Supplement (50X), serum free | 10ml |
GIBCO | 17502048 | N-2 Supplement (100X) | 5ml |
GIBCO | A1049101 | RPMI 1640 Medium (ATCC Modification) | 500ml |
GIBCO | 35050061 | GlutaMAX Supplement | 100ml |
GIBCO | 35050079 | GlutaMAX? Supplement | 20 x 100 mL |
GIBCO | A3021002 | CTS? AIM V? SFM (RUO) | 1000ml |
GIBCO | 12440061 | IMDM | 10*500ml |
GIBCO | 12440053 | IMDM | 500ml |
GIBCO | 31985070 | Opti-MEM I Reduced Serum Medium | 500ml |
GIBCO | 31985088 | Opti-MEM I Reduced Serum Medium | 10*500ml |
GIBCO | 21127022 | Ham's F-12K (Kaighn's) Medium | 500ml |
GIBCO | 21127030 | Ham's F-13K (Kaighn's) Medium | 10*500ml |
GIBCO | 14190144 | DPBS, no calcium, no magnesium | 500ml |
GIBCO | 14190250 | DPBS, no calcium, no magnesium | 10*500ml |
GIBCO | 11095080 | MEM | 500ml |
GIBCO | 11095080 | MEM | 10*500ml |
GIBCO | 25030164 | L-Glutamine (200 mM) | 20 x 100 mL |
GIBCO | 25030081 | L-Glutamine (200 mM) | 100 mL |
GIBCO | 10687010 | Hygromycin B (50 mg/mL) | 20 mL |
GIBCO | C10228 | Countess? Cell Counting Chamber Slides | 50 slides |
GIBCO | C10315 | Countess? Cell Counting Chamber Slides | 500slides |
GIBCO | 12338018 | FreeStyle? 293 Expression Medium | 1,000 mL |
GIBCO | 12338026 | FreeStyle? 293 Expression Medium | 6*1,000 mL |
GIBCO | 10902088 | Sf-900?II SFM | 1,000 mL |
GIBCO | 10902104 | Sf-901?II SFM | 6*1,000 mL |
GIBCO | 41400045 | Insulin-Transferrin-Selenium (ITS -G) (100X) | 10 mL |
GIBCO | 51500056 | Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) | 10 mL |
GIBCO | 51300044 | Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) | 10 mL |
GIBCO | 18080044 | SuperScript? III Reverse Transcriptase | 10000U |
GIBCO | 18080051 | SuperScript? III First-Strand Synthesis System | 50T |
GIBCO | 13778150 | Lipofectamine? RNAiMAX Transfection Reagent | 1.5ml |
GIBCO | 15250061 | Trypan Blue Solution, 0.4% | 100ml |
GIBCO | R25001 | Zeocin? Selection Reagent | 8*1.25ml |
GIBCO | 10687010 | Hygromycin B (50 mg/mL) | 20ml |
GIBCO | 31800022 | RPMI 1640 Medium, powder | 10*1L |
GIBCO | 12800017 | DMEM, powder, high glucose, pyruvate | 10*1L |
GIBCO | 12100046 | DMEM, powder, high glucose | 10*1L |
GIBCO | A1113803 | Puromycin Dihydrochloride | 10*1ml |
GIBCO | A1113903 | Blasticidin S HCl (10 mg/mL) | 10*1ml |
GIBCO | Q32854 | Qubit? dsDNA HS Assay Kit | 500 assays |
GIBCO | Q32856 | Qubit? Assay Tubes | 500 tubes |
GIBCO | A14524 | ExpiFectamine? 293 Transfection Kit | For 1 L of culture |
GIBCO | A1435101 | Expi293? Expression Medium | 1,000 mL |
GIBCO | A1435102 | Expi293? Expression Medium | 6*1,000mL |
GIBCO | A29129 | ExpiFectamine? CHO Transfection Kit | For 1 L of culture |
GIBCO | A2910001 | ExpiCHO? Expression Medium | 1000 mL |
GIBCO | A2910002 | ExpiCHO? Expression Medium | 6*1,000mL |
GIBCO | 10010023 | PBS, pH 7.4 | 500ml |
GIBCO | 10010049 | PBS, pH 7.5 | 10*500ml |
GIBCO | 11875093 | RPMI 1640 Medium | 500ml |
GIBCO | 11875119 | RPMI 1640 Medium | 10*500ml |
GIBCO | 11965092 | DMEM, high glucose | 500ml |
GIBCO | 11965118 | DMEM, high glucose | 10*500ml |
GIBCO | 11995073 | DMEM, high glucose, pyruvate | 10*500ml |
GIBCO | 11995065 | DMEM, high glucose, pyruvate | 500ml |
GIBCO | Q32854 | Qubit? dsDNA HS Assay Kit | 500 assays |
GIBCO | Q32856 | Qubit? Assay Tubes | 500 tubes |
GIBCO | 31800022 | RPMI 1640 Medium, powder | 10*1L |
GIBCO | 12800017 | DMEM, powder, high glucose, pyruvate | 10*1L |
GIBCO | 12100046 | DMEM, powder, high glucose | 10*1L |
GIBCO | 12338018 | FreeStyle? 293 Expression Medium | 1,000 mL |
GIBCO | 12338026 | FreeStyle? 293 Expression Medium | 6*1,000 mL |
GIBCO | 17100017 | Collagenase, Type I, powder | 1g |
GIBCO | 17101015 | Collagenase, Type II, powder | 1g |
GIBCO | 17104019 | Collagenase, Type IV, powder | 1g |
GIBCO | A1148301 | CD FortiCHO? | 1000ml |
GIBCO | 10743029 | CD CHO AGT? | 1000ml |
GIBCO | A2661501 | Dynamis? | 1000ml |
GIBCO | 12563029 | TrypLE? Select | 500ML/瓶 |
GIBCO | 12309050 | OptiPRO? SFM | 100ML/瓶 |
GIBCO | 0010057DG | 抗結團劑 | 100ml/瓶 |
GIBCO | 0010057AE | 抗結團劑 | 20ml/瓶 |
GIBCO | 10099141C | 胎牛血清 | 500ml |
GIBCO | 16010142 | 新生牛血清 | 1000ml |
GIBCO | 0025062EJ | DMEM | 100L/桶干粉 |
GIBCO | 0835048SA | DMEM | 100L/桶干粉 |
GIBCO | 0035008EJ | M199 | 100L/桶干粉 |
GIBCO | 21051040 | 谷氨酰胺 | 100L/桶干粉 |
GIBCO | 27250018 | 胰酶 | 100g干粉 |
GIBCO | 12500096 | DMEM/F-12 | 50L/桶干粉 |
GIBCO | A2503104 | EfficientFeed? C+ AGT? | 1L/桶干粉 |
GIBCO | A2503105 | EfficientFeed? C+ AGT? | 10L/桶干粉 |
GIBCO | 12490025 | CD CHO AGT? | 10L/桶干粉 |
GIBCO | 0895086IN | 水解乳蛋白 | 50L/桶干粉 |
GIBCO | 11800042 | 水解乳蛋白 | 5KG/桶干粉 |
GIBCO | 40203D | 磁珠 | 10ml/瓶 |
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